Effects of interferon-a and interferon-b on development of antigen-specific B cells and hybridoma generation (38.28)
| Autor: | Sandra Santulli-Marotto, Ted Petley, Jamie Fisher, Jennifer Pitcher, Michael Rycyzyn, Jill Giles-Komar |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 182:38.28-38.28 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.182.supp.38.28 |
| Popis: | The ratio of Interferon-α (IFN-α) and Interferon-β (IFN-β) produced during an immune response can significantly impact the outcome. We find that administration of IFN-α and IFN-β during immunization can alter the fate of antigen-specific B cells with minimum affect on antibody titers in mice. In vitro stimulation with IFN-α and IFN-β either individually or in combination triggered variations in interferon-responsive gene expression that directly correlated to the activity level of the individual interferons as well as the ratio of IFN-α to IFN-β. However, when the activity of IFN-α and IFN-β was normalized according to gene expression profiles the outcome of immunizing mice with the same antigen, using the same protocol but varying relative ratios of IFN-α and IFN-β yielded little to no antigen-specific B cells. Though animals could produce nearly identical antigen-specific antibody titers there was an average difference of almost 7- fold in terms of IgG producing hybridomas and almost 100-fold difference in the number of antigen-specific clones. Importantly, not all genes were affected equally and the in vitro data were generated using a cell line derived from a different mouse strain than used for the immunization studies. Perhaps a subset of genes induced by interferons plays an important role in B cell activation and development or the B cell response to IFN-α and IFN-β varies depending on the strain of mouse. |
| Databáze: | OpenAIRE |
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