Engineering Trichosporon oleaginosus for enhanced production of lipid from volatile fatty acids as carbon source
| Autor: | Kyungsoo Lee, Ki Jun Jeong, Yong Jae Lee, Ho Nam Chang |
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| Rok vydání: | 2019 |
| Předmět: |
Strain (chemistry)
Bioconversion General Chemical Engineering Mutagen 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology medicine.disease_cause Oleic acid chemistry.chemical_compound Food waste 020401 chemical engineering chemistry medicine lipids (amino acids peptides and proteins) Fermentation Stearic acid Food science 0204 chemical engineering 0210 nano-technology Fatty acid methyl ester |
| Zdroj: | Korean Journal of Chemical Engineering. 36:903-908 |
| ISSN: | 1975-7220 0256-1115 |
| DOI: | 10.1007/s11814-018-0229-7 |
| Popis: | Trichosporon oleaginosus is one of the most promising hosts for microbial lipid production owing to its high-productivity; In an effort to develop an economical production process, we engineered T. oleaginosus towards high-lipid production from volatile fatty acids (VFA) derived from anaerobic fermentation of food waste. First, we established a method for labeling intracellular lipid with lipophilic BODIPY fluorescent dye. Next, a random library was constructed by treatment with a chemical mutagen, and high-lipid producers were screened using fluorescence-activated cell sorting. Subsequently, one clone, N14, was successfully isolated, which exhibited 3-fold higher lipid production (19.4%) in VFA (6 g/L) media than the wild-type strain, and also showed increased lipid production in higher concentrations of VFA (18 or 24 g/L). Based on fatty acid methyl ester (FAME) analysis, N14 contained higher stearic acid (C18:0) and oleic acid (C18:1) content compared with those of the wild-type strain. |
| Databáze: | OpenAIRE |
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