Effect of Methyl Jasmonate and Mannitol Application on Growth and Eugenol Content in Callus Cultures of Carnation
| Autor: | Moemen S. Hanafy, Mohamed A. Matter, Usama I. Aly |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Methyl jasmonate Plant Science Carnation Biology biology.organism_classification 01 natural sciences Plant tissue High-performance liquid chromatography Eugenol chemistry.chemical_compound Horticulture chemistry Callus medicine Mannitol 010606 plant biology & botany Biotechnology medicine.drug |
| Zdroj: | Plant Tissue Culture and Biotechnology. 27:227-240 |
| ISSN: | 1818-8745 1817-3721 |
| Popis: | A protocol for callus induction from leaves of carnation is established. MS fortified with 2.0 mg /l 2,4‐D and 0.5 mg/l Kn was found to be the appropriate medium for producing callus cultures. Methyl jasmonate at 20 μM concentration showed the highest growth rate (98.21 mg/day) while mannitol concentrations at 2% gave the peak growth rate (97.1 mg/day).The highest content of eugenol (14.65 μg/100 g calli) was recorded with medium containing 20 μM methyl jasmonate followed by 8.16 μg/100 g calli with 10 μM methyl jasmonate. In presence of 0.5% mannitol, maximum eugenol content (18.00 μg/100 g calli) was obtained.Plant Tissue Cult. & Biotech. 27(2): 227-240, 2017 (December) |
| Databáze: | OpenAIRE |
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