Pathogenicity of Vibrio anguillarum for Juvenile Winter Flounder, Pseudopleuronectes americanus
| Autor: | R. E. Wolke, W. D. Watkins, V. J. Cabelli |
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| Rok vydání: | 1981 |
| Předmět: | |
| Zdroj: | Canadian Journal of Fisheries and Aquatic Sciences. 38:1045-1051 |
| ISSN: | 1205-7533 0706-652X |
| DOI: | 10.1139/f81-144 |
| Popis: | The pathogenicity of Vibrio anguillarum for juvenile winter flounder, Pseudopleuronectes americanus, was quantitatively established under laboratory conditions. Fish were obtained from Rhode Island waters, acclimatized in tanks for 4–6 wk, and then challenged with V. anguillarum. The LD50 was relatively constant for 24–to 72-h cultures of the organism using intraperitoneal, intramuscular, and intravenous challenge routes. The LD50 via oral inoculation was about 3 logs greater. The LD50 for fish collected, acclimatized, and challenged during the winter was ~3 logs less than that found for fish in the summer. The median intramuscular LD50 for 11 strains examined under the optimum conditions for infection was 2.4 × 105 (range 7.3 × 104 to 1.6 × 106) organisms. Gross and histopathological changes in the acute form of the disease were minimal, being limited to anemia, leucopenia, ecchymosis and necrosis at the site of inoculation, and behavioral changes. A chronic form of vibriosis was observed primarily during the summer in fish given sublethal doses. A potent, lethal V. anguillarum toxin could not be demonstrated. Potentially, this reproducible host–parasite system might have use in a short-term bioassay procedure for toxic pollutants of the marine environment.Key words: Vibrio anguillarum, winter founder, LD50, histopathological changes, acute and chronic vibriosis, toxin, bioassay |
| Databáze: | OpenAIRE |
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