Human Lymph Nodes Maintain TCF-1hi Memory T Cells with High Functional Potential and Clonal Diversity throughout Life
| Autor: | Yufeng Shen, Uri Hershberg, Wenzhao Meng, Aaron M. Rosenfeld, Takashi Senda, Bochao Zhang, Dora Chen, Dustin J. Carpenter, Adeeb Rahman, Eline T. Luning Prak, Amy L. Friedman, Tomer Granot, Brahma V. Kumar, Michelle Miron, Harvey Lerner, Donna L. Farber |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
T cell Immunology T-cell receptor Biology Stem cell marker CXCR4 Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Lymphatic system Antigen 030220 oncology & carcinogenesis medicine Immunology and Allergy Bone marrow CD8 |
| Zdroj: | The Journal of Immunology. 201:2132-2140 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Translating studies on T cell function and modulation from mouse models to humans requires extrapolating in vivo results on mouse T cell responses in lymphoid organs (spleen and lymph nodes [LN]) to human peripheral blood T cells. However, our understanding of T cell responses in human lymphoid sites and their relation to peripheral blood remains sparse. In this study, we used a unique human tissue resource to study human T cells in different anatomical compartments within individual donors and identify a subset of memory CD8+ T cells in LN, which maintain a distinct differentiation and functional profile compared with memory CD8+ T cells in blood, spleen, bone marrow, and lungs. Whole-transcriptome and high-dimensional cytometry by time-of-flight profiling reveals that LN memory CD8+ T cells express signatures of quiescence and self-renewal compared with corresponding populations in blood, spleen, bone marrow, and lung. LN memory T cells exhibit a distinct transcriptional signature, including expression of stem cell–associated transcription factors TCF-1 and LEF-1, T follicular helper cell markers CXCR5 and CXCR4, and reduced expression of effector molecules. LN memory T cells display high homology to a subset of mouse CD8+ T cells identified in chronic infection models that respond to checkpoint blockade immunotherapy. Functionally, human LN memory T cells exhibit increased proliferation to TCR-mediated stimulation and maintain higher TCR clonal diversity compared with memory T cells from blood and other sites. These findings establish human LN as reservoirs for memory T cells with high capacities for expansion and diverse recognition and important targets for immunotherapies. |
| Databáze: | OpenAIRE |
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