Complete mitochondrial genome sequences of Brassica rapa (Chinese cabbage and mizuna), and intraspecific differentiation of cytoplasm in B. rapa and Brassica juncea
| Autor: | Hiroshi Yamagishi, Kaori Nishimura, Saki Hatono, Mai Tsujimura, Yoko Murakami |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA Brassica food and beverages Single-nucleotide polymorphism Plant Science Biology biology.organism_classification Genome Japonica 03 medical and health sciences Complete sequence 030104 developmental biology Brassica rapa Botany Genetics Ploidy Agronomy and Crop Science |
| Zdroj: | Breeding Science. 67:357-362 |
| ISSN: | 1347-3735 1344-7610 |
| DOI: | 10.1270/jsbbs.17023 |
| Popis: | The complete sequence of the mitochondrial genome was determined for two cultivars of Brassica rapa. After determining the sequence of a Chinese cabbage variety, 'Oushou hakusai', the sequence of a mizuna variety, 'Chusei shiroguki sensuji kyomizuna', was mapped against the sequence of Chinese cabbage. The precise sequences where the two varieties demonstrated variation were ascertained by direct sequencing. It was found that the mitochondrial genomes of the two varieties are identical over 219,775 bp, with a single nucleotide polymorphism (SNP) between the genomes. Because B. rapa is the maternal species of an amphidiploid crop species, Brassica juncea, the distribution of the SNP was observed both in B. rapa and B. juncea. While the mizuna type SNP was restricted mainly to cultivars of mizuna (japonica group) in B. rapa, the mizuna type was widely distributed in B. juncea. The finding that the two Brassica species have these SNP types in common suggests that the nucleotide substitution occurred in wild B. rapa before both mitotypes were domesticated. It was further inferred that the interspecific hybridization between B. rapa and B. nigra took place twice and resulted in the two mitotypes of cultivated B. juncea. |
| Databáze: | OpenAIRE |
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