Studies on plant gums. Proteases in neem (Azadirachta indica) gum
| Autor: | T. N. Pattabiraman, B. Ramakrishna Nayak, N. Mallikarjuna Rao |
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| Rok vydání: | 1979 |
| Předmět: |
Exudate
chemistry.chemical_classification Chromatography biology Plant Gums Albumin General Medicine Azadirachta biology.organism_classification General Biochemistry Genetics and Molecular Biology Enzyme assay Hydrolysis Enzyme chemistry Casein medicine biology.protein medicine.symptom General Agricultural and Biological Sciences |
| Zdroj: | Journal of Biosciences. 1:393-400 |
| ISSN: | 0973-7138 0250-5991 |
| DOI: | 10.1007/bf02704621 |
| Popis: | Proteolytic activity was detected in neem (Azadirachta indica) exudate gum when tested with casein and albumin as substrates. The enzyme activity was separated into two fractions by chromatography on TEAE-cellulose after EDTA treatment. Both the enzyme fractions were fairly stable to high temperatures and wide range of pH conditions. The pH optima were found to be around 6.5. Phenylmethyl sulphonylfluoride inhibited the activity of both the fractions. EDTA, Β-mercaptoethanol, tosylamide phenylethylchloromethylketone, tosyllysine chloroimethylketone,p-chloromercuribenzoate and dithiobis-2-nitrobenzoie acid did not affect the activity of the two enzyme fractions. The two fractions had no hydrolytic action on a variety of synthetic substrates tested. |
| Databáze: | OpenAIRE |
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