| Popis: |
Evaluate expression and amplification of EGFR in cholangiocarcinoma (CCA) and correlate with the different histological types. 74 patients with CCA from 1992 to 2017 were evaluated. Cases were classified in large duct subtype (DL), cholangiolocarcinoma (CLC), intermediate cell carcinoma (ICC) and papillary (LP).The immunohistochemistry (IHQ) was conducted in 71 cases and the amplification of EGFR was using the fluorescence in situ hybridization (FISH) in 48 cases. From the 74 patients, most lesions affected the perihilar topography (54%, 40/74), extrahepatic portion (27%, 20/74) and the least frequent was the intrahepatic (19%, 14/74). Periductal infiltrative macroscopic growth patterns 60.9% (45/74) and the mass forming 33.7% (25/74) were the predominant, intraductal pattern 5.4% (4/74) lower frequency. The DL subtype was the most frequent (66.2%, 49/74), followed by the CLC (21.7%, 16/74). The LP (8.1%, 6/74) and the ICC (4.0%, 3/74) had a lower frequency. In the IHQ, EGFR showed positivity in 80.2% (57/71), presenting moderate intensity 2+ in 55.0% (39/71) of the cases and strong intensity 3+ in 25.3% (18/71), 14 were detected as negative 19.8%. The FISH, of the 48 cases, 10.5% (5/48) were amplified by the gain in the number of copies of the EGFR gene and 89.5% (43/48) were considered negative. The amplified cases were distributed in 12.5% (4/32) of the DL subtype and 12.5% (1/8) of the CLC subtype. The IHQ expression of EGFR in the tumor is high in all histological subtypes of CCA. EGFR amplification occurred in a small portion of the DL and CLC subtypes. |
