Production of an endo-polygalacturonate trans-eliminase by a potato dry-rot pathogen, Fusarium roseum ‘Avenaceum’, in culture and in diseased tissue

Autor:	D.F. Bateman, Jaqueline M. Mullen
Rok vydání:	1971
Předmět:	chemistry.chemical_classification Isoelectric focusing fungi food and beverages Plant Science Biology Solanum tuberosum Enzyme Isoelectric point chemistry Biochemistry Sephadex Genetics Extracellular Maceration (wine) Food science Dry rot
Zdroj:	Physiological Plant Pathology. 1:363-373
ISSN:	0048-4059
DOI:	10.1016/0048-4059(71)90056-7
Popis:	Fusarium roseum (Lk.) Snyd. and Hans. ‘Avenaceum’, which causes a dry rot of Solanum tuberosum L., produces an extracellular endo-polygalacturonate trans -eliminase when cultured on a potato broth-pectic acid medium or on intact potato tubers. This enzyme is calcium dependent and exhibits maximum activity between pH 9·0 and 9·5. It has been purified 117-fold by ammonium sulfate fractionation and electrofocusing (pH range 7 to 10), and behaves as a single component with an isoelectric point of pH 8·2. It has a molecular weight of c . 30·5 × 10 3 as determined by sucrose density gradient centrifugation and gel filtration in Sephadex G-75. Both crude and purified preparations of the enzyme cause maceration and cell death of potato tuber tissue.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d0e631372bb6ee5d8a67006a087b479f https://doi.org/10.1016/0048-4059(71)90056-7 Zobrazit plný text záznamu