Cytokine-inducing and anti-inflammatory activity of chitosan and its low-molecular derivative
| Autor: | A. A. Kalitnik, Pavel A. Markov, V. N. Davydova, Sergey V. Popov, A V Volod'ko, I M Ermak |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.drug_class medicine.medical_treatment 02 engineering and technology Pharmacology Polysaccharide Applied Microbiology and Biotechnology Biochemistry Anti-inflammatory Chitosan 03 medical and health sciences chemistry.chemical_compound Blood serum medicine chemistry.chemical_classification biology 021001 nanoscience & nanotechnology In vitro 030104 developmental biology Cytokine chemistry Myeloperoxidase biology.protein Tumor necrosis factor alpha 0210 nano-technology |
| Zdroj: | Applied Biochemistry and Microbiology. 52:476-482 |
| ISSN: | 1608-3024 0003-6838 |
| Popis: | A low-molecular derivative of the polysaccharide (5 kDa) was obtained and its cytokine-inducing and anti-inflammatory activity was studied by free radical depolymerization of chitosan (110 kDa). It was shown that high-molecular chitosan in vitro inhibited the synthesis of anti-inflammatory cytokine, the tumor necrosis factor alpha induced by endotoxin. In the case of peroral introduction to experimental animals, high- and low-molecular chitosans stimulated synthesis of the anti-inflammatory cytokine IL-10 in the blood serum of mice; in this case, the activity of the high-molecular derivative was two times higher as compared with the initial polysaccharide. With peroral introduction, the initial polysaccharide (50 mg/kg) and its derivative inhibited the development of chemically induced inflammation of experimental animals’ large intestines, which was manifested as a decrease in the affected area and the degree of damage to the large intestine wall, as well as a two-fold reduction of myeloperoxidase activity. According to morphological and biochemical characteristics, the effect of chitosans was similar to that of a hormone anti-inflammatory drug, prednisolone. |
| Databáze: | OpenAIRE |
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