Molecular detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies: a prospective evaluation of RIDA®GENE Helicobacter pylori assay
| Autor: | Pieter-Jan Cuyle, Claire Bourgain, Bea Van den Poel, Paul Christiaens, Veerle Moons, Johan Frans, Saskia Carton, Peter Bossuyt, Annick Smismans, Isabel Micalessi, Gust Van Olmen, Sarah Gils |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
biology business.industry General Medicine Helicobacter pylori biology.organism_classification Gastroenterology Prospective evaluation Empirical treatment 03 medical and health sciences 0302 clinical medicine Real-time polymerase chain reaction 030220 oncology & carcinogenesis Internal medicine Clarithromycin Clarithromycin resistance medicine Immunohistochemistry 030212 general & internal medicine business Gene medicine.drug |
| Zdroj: | Acta Clinica Belgica. 76:177-183 |
| ISSN: | 2295-3337 1784-3286 |
| DOI: | 10.1080/17843286.2019.1685741 |
| Popis: | Background: Empirical treatment of Helicobacter pylori (HP) depends on the local prevalence of clarithromycin resistance but data are lacking and culturing of HP is time-consuming. We evaluated RIDA®GENE Helicobacter pylori assay (r-biopharm), a quantitative PCR assay for detecting HP and clarithromycin resistance mutations in gastric biopsies.Material/methods: Gastric biopsies were obtained from each of 436 consecutive patients referred for gastroscopic investigation and results of qPCR were compared to culture and immunohistochemical staining (IHCS).Results: Of 436 samples, 47 were positive for HP by PCR (11%), 42 by culture (9.7%) and 44 by IHCS (10%). Compared to culture, sensitivity and specificity of the qPCR were 100% and 99%, respectively, and 96% and 99% compared to IHCS. The sensitivity and specificity for clarithromycin resistance detection were 92% and 97%, respectively.Conclusions: RIDA®GENE Helicobacter pylori assay reliably and rapidly detects HP and its resistance to clarithromycin in human gastric biopsies. |
| Databáze: | OpenAIRE |
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