Regulation of Surface Free Thiol Levels During CD8+ T Cell Activation, Proliferation and Differentiation (132.7)
| Autor: | Samuel Pellom, Ryan Michalek, Jason Grayson |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 184:132.7-132.7 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Upon both B and T cell activation the level of reactive oxygen intermediates (ROI) increases. Prior studies in the literature demonstrated that activated lymphocytes have increased total levels of surface free thiols, potentially as a compensatory mechanism to prevent surface oxidation. In this study, we determined how surface free thiol levels were regulated during the activation, proliferation, and differentiation of naïve CD8+ T cells both in vitro and in vivo. In vitro, we found that the levels of surface free thiols increased within 24 hours regardless of the activation stimulus and were maintained for at least three divisions. To understand how levels were modulated in vivo, we examined antigen-specific CD8+ T cells using lymphocytic choriomeningitis virus (LCMV) infection of mice and MHC Class I tetramers. On day 5 postinfection, the levels of surface free thiols were 8-fold higher on effector cells compared to those found on naïve CD8+ T cells. By day 8 postinfection this had decreased to 4-fold. After the infection was cleared, memory CD8+ T cells had 1.5-fold higher levels of surface free thiols than naïve cells. This contrasted with the results during chronic LCMV-Clone 13 infection. On day 8 postinfection surface free thiol levels were 11-fold higher and remained elevated until day 35 postinfection. These results suggest that surface free thiol levels are elevated most highly in rapidly proliferating cells and do not correlate with lymphocyte function. |
| Databáze: | OpenAIRE |
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