| Popis: |
Biochemical changes during ovarian growth and development have been studied in a wild type strain of the housefly, Musca domestica, where both intra- and inter-ovarian synchronous development can be achieved. At 29°C, the first gonotrophic cycle is complete 72 hr after eclosion, and the second cycle in an additional 40 hr. In the first cycle, ovarian DNA content increases rapidly between 48 to 60 hr after emergence. RNA content begins rising at 36 hr and reaches a maximum of 180 μg/ovary. In vitro labeling of ovaries with 3H-uridine indicates that ribosomal RNA is the major species of RNA present during the previtellogenic and early vitellogenic stages. Changes in protein content are similar to those in DNA content. Analysis of active ribosomes indicates that ovarian protein synthesis peaks 48 hr after eclosion, and represents the comboned activity of follicle cells, nurse cells and oocyte. |
