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This exploration work target was to upgrade the micropropagation of potato cultivars through the utilizationof new light sources in the development rooms. The experiment consisted of two potato cultivars (Diamant and agria),and five light sources (white, blue, green and red LEDs; and white fluorescent lights). The explants comprised of nodalsegments containing one bud, segregated from plantlets developed in vitro. The experimental design was completelyrandomized arranged in a 2x5 factorial, with 4 replications. Each experimental unit consisted of a flask with fiveexplants. Three 28-day successive subcultures were done in MS medium and incubated under controlled conditions(temperature = 25+2oC; photoperiod = 16 hours; light intensity = 20 μmol m-2 s-1). Toward the finish of everysubculture, plantlet length, number of nodes per plantlet, internode length and plantlet fresh weight were assessed. Afterthe second subculture, differences were found among the two potato cultivars developed in vitro: "Agria" performedbetter than "Diamant" in most plantlet growth parameters. Different light sources influenced the potato plantletdevelopment: red and green LEDs were the most and least prescribed for plantlet advancement respectively, in view ofthe after effects of bud number per plantlet, plantlet length. Photosynthetic pigment contents were significantly differentunder the different light treatments. Chlorophyll a, b and carotenoids were higher in plantlets grown under Red, and lessin plantlets under green LED light. |
