Creation and Characterization of a Double Null Puroindoline Genotype in Spring Wheat
| Autor: | John M. Martin, Andrew C. Hogg, Richard W. Webster, Michael J. Giroux |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Ethyl methanesulfonate Organic Chemistry Haplotype food and beverages Locus (genetics) Biology 01 natural sciences Null allele 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Genetic linkage Backcrossing Genotype Allele 010606 plant biology & botany Food Science |
| Zdroj: | Cereal Chemistry Journal. 94:805-810 |
| ISSN: | 0009-0352 |
| DOI: | 10.1094/cchem-04-17-0071-rw |
| Popis: | Wheat (Triticum aestivum L.) grain hardness is controlled by the Ha locus, which is composed of two closely linked genes, Puroindoline a (Pina) and Puroindoline b (Pinb). Hard grain results from mutations in either of the Pin genes. Previous results have shown that the Pina-D1b (Pina null) allele has harder grain than other naturally occurring Pin alleles. Our goal was to create, identify, and characterize a double null Pin genotype by identifying a Pinb null mutation in a variety carrying the Pina-D1l null allele. Seeds of Fortuna, which has a premature stop codon in Pina, were treated with ethyl methanesulfonate. Two premature stop codon mutations were identified in Pinb using direct sequencing. The double null Pin haplotype was characterized after backcrossing to the parent variety Fortuna to create Pina null populations segregating for the presence of Pinb. The double null group was 6 units harder than the single null with no difference in other kernel characteristics. The milling characteristics diff... |
| Databáze: | OpenAIRE |
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