Helicobacter pylori infection induces accumulation of Vdelta1 T cells via CCR2 upregulation
| Autor: | Kenji Suzuki, Kazumasa Miyake, Choitsu Sakamoto, Nobue Ueki, Tomotaka Shindo, Tatsuhiko Hamamoto, Tetsuro Hiratsuka, Masanori Kusunoki, Taku Tsukui, K. Ohashi, K. Gudis, Seiji Futagami, Ken Wada |
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| Rok vydání: | 2006 |
| Předmět: |
CCR2
biology biology.organism_classification In vitro medicine.anatomical_structure Downregulation and upregulation Immunology Gastric mucosa medicine Pharmacology (medical) Helicobacter General Pharmacology Toxicology and Pharmaceutics Gastritis medicine.symptom CC chemokine receptors Immunostaining |
| Zdroj: | Alimentary Pharmacology Therapeutics Symposium Series. 2:8-15 |
| ISSN: | 1746-6342 1746-6334 |
| DOI: | 10.1111/j.1746-6342.2006.00020.x |
| Popis: | Summary Background We investigated factors that impact γδ T-cell phenotype accumulation in Helicobacter pylori-infected gastric mucosa and peripheral blood. Aim To determine whether H. pylori infection induces accumulation of Vδ1 T cells via CC chemokine receptor 2 (CCR2) upregulation. Methods Mucosal biopsy samples from 22 H. pylori-free and 75 H. pylori-infected patients were classified into grades I–III gastritis groups as per our previous study. The number of γδ, Vδ1 and Vδ2 T cells was evaluated by immunostaining and then compared with counts in 17 patients after H. pylori eradication. TGF-β, IFN-γ and CCR2 mRNA expression levels in Vδ1 T cells stimulated by H. pylori component were also evaluated. Results γδ T-cell count was significantly higher in grade III gastritis patients, who exhibited strong IgA and IgG responses to H. pylori urease, than in other groups. Vδ1 T cells were found dominantly residing in H. pylori-infected gastric mucosa, whereas Vδ2 T cells were mainly found in peripheral blood. Vδ1 T-cell count was significantly reduced after H. pylori eradication therapy. In in vitro studies, H. pylori component stimulation significantly upregulated both TGF-β and IFN-γ production in supernatant from stimulated Vδ1 T cells. Moreover, CCR2 mRNA expression in Vδ1 T cells stimulated with H. pylori components was significantly increased. Conclusion Accumulation of Vδ1 T cells may occur through the upregulation of CCR2 expression. |
| Databáze: | OpenAIRE |
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