Identification and characterization of flavonoids in the root exudate of Robinia pseudoacacia

Autor: Petra Scheidemann, Astrid Wetzel
Rok vydání: 1997
Předmět:
Zdroj: Trees. 11:316-321
ISSN: 1432-2285
0931-1890
DOI: 10.1007/pl00009677
Popis: Eight compounds exuded from young roots of black locust (Robinia pseudoacacia) were separated by two-dimensional HPTLC, by HPLC and GC, and were identified by spectroscopic methods (ultraviolet/visible spectroscopy and mass spectrometry) as 4′,7-dihydroxyflavone, apigenin, naringenin, chrysoeriol and isoliquiritigenin. Structural assignments were confirmed by comparison with authentic standards. The capacity to induce β-galactosidase activity in Rhizobium sp. NGR234 containing a nod box::lacZ fusion on plasmid pA27 identified these flavonoids and the chalcone as nod gene inducers. This indicates the important role of these compounds in nodulation of this legume tree.
Databáze: OpenAIRE