Identification and characterization of flavonoids in the root exudate of Robinia pseudoacacia
Autor: | Petra Scheidemann, Astrid Wetzel |
---|---|
Rok vydání: | 1997 |
Předmět: | |
Zdroj: | Trees. 11:316-321 |
ISSN: | 1432-2285 0931-1890 |
DOI: | 10.1007/pl00009677 |
Popis: | Eight compounds exuded from young roots of black locust (Robinia pseudoacacia) were separated by two-dimensional HPTLC, by HPLC and GC, and were identified by spectroscopic methods (ultraviolet/visible spectroscopy and mass spectrometry) as 4′,7-dihydroxyflavone, apigenin, naringenin, chrysoeriol and isoliquiritigenin. Structural assignments were confirmed by comparison with authentic standards. The capacity to induce β-galactosidase activity in Rhizobium sp. NGR234 containing a nod box::lacZ fusion on plasmid pA27 identified these flavonoids and the chalcone as nod gene inducers. This indicates the important role of these compounds in nodulation of this legume tree. |
Databáze: | OpenAIRE |
Externí odkaz: |