| Autor: |
Michael G. Hobart, Vido Ramassar, Nelson Goes, Philip F. Halloran |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Transplantation. 62:1901-1907 |
| ISSN: |
0041-1337 |
| DOI: |
10.1097/00007890-199612270-00038 |
| Popis: |
The class II transactivator (CIITA) is a protein that induces the transcription of MHC class II genes. We studied the expression of CIITA in vivo, comparing steady state levels of CIITA and class II mRNA in various mouse tissues. Many tissues in normal mice contained mRNA for CIITA, correlating with class II mRNA. The basal expression of CIITA and class II mRNA in mice with disrupted IFN-γ genes (GKO mice) was similar to that in wild-type mice. Injection of rIFN-γ strongly induced CIITA and class II mRNA: CIITA mRNA increased at 2 hr and declined to baseline by 48 hr, whereas class II mRNA increased at 24 hr and returned to baseline at 7 days. Proinflammatory stimuli that induce IFN-γ production (allogeneic cells and LPS) induce CIITA and class II expression in wild-type mice, but not in GKO mice. CIITA induction by IFN-γ was partially sensitive to cycloheximide, suggesting that another protein is required for CIITA induction. The data suggest that CIITA is a major regulator of basal and induced class II expression in vivo. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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