Antioxidant property of a Thai traditional formula for longevity
| Autor: | Penchom Peungvicha, S. Nakornchai, Y. Wongkrajang, Rungravi Temsiririrkkul, S Luanchoy, S Tiangkul |
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| Rok vydání: | 2009 |
| Předmět: |
Pharmacology
Piper Antioxidant biology Traditional medicine DPPH medicine.medical_treatment Organic Chemistry Pharmaceutical Science Streblus asper medicine.disease biology.organism_classification Hemolysis Analytical Chemistry chemistry.chemical_compound Complementary and alternative medicine chemistry Phytochemical Drug Discovery medicine Molecular Medicine Trolox Cyperus rotundus |
| Zdroj: | Planta Medica. 75 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0029-1234482 |
| Popis: | Antioxidant activity of a Thai traditional formula for longevity was studied. The formula comprised six herbs, as follows: Albizia procera, Cyperus rotundus, Diospyros rhodocalyx, Piper nigrum, Tinospora crispa and Streblus asper. Each components and the formula were separately extracted by 95% ethanol and concentrated using a reduced-pressure evaporator. The antioxidant properties were detected by DPPH method using vitamin C and Trolox as reference standards. In vitro oxidative hemolysis of sheep red blood cells was used as a model to study the free radical-induced damage of biological membranes by AAPH. From the DPPH method, Albizia procera extract demontrated the most potent antioxidant properties (IC 50 44.34 µg/ml), while vitamin C and Trolox had IC 50 of 17.47 µg/ml and 22.75 µg/ml, respectively. From the AAPH hemolysis method, Albizia procera, the formula for longevity, Cyperus rotundus, Diospyros rhodocalyx, Piper nigrum, Tinospora crispa and Streblus asper extracts at a concentration of 5 mg/ml could prolong the time of 50% hemolysis from 78 min to 157, 142, 126, 114, 108, 101 and 100 min, respectively, while the time of 50% hemolysis of Trolox at a concentration of 0.5 mg/ml was 160 min. Phytochemical screening tests showed the presence of phenolic compounds, tannins and flavonoids in the formula Cyperus rotundus and Albizia procera; and phenolic compounds and flavonoids in Piper nigrum, Diospyros rhodocalyx and Streblus asper. Tinospora crispa extract contained only phenolic compounds. Keyword: Albizia procera, Antioxidant activities, Cyperus rotundus, Diospyros rhodocalyx, DPPH method, Piper nigrum, Streblus asper, Thai traditional formula for longevity, Tinospora crispa, 50% hemolysis |
| Databáze: | OpenAIRE |
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