Expression of Human Bone Morphogenetic Proteins-2 or -4 in Murine Mesenchymal Progenitor C3H10T½ Cells Induces Differentiation into Distinct Mesenchymal Cell Lineages

Autor:	Thomas Ankenbauer, Angela Hollnagel, Gerhard Gross, Dietmar Schröder, Hubert Mayer, Marion Ahrens
Rok vydání:	1993
Předmět:	animal structures Parathyroid hormone receptor Mesenchymal stem cell Osteoblast Cell Biology General Medicine Transfection Biology Molecular biology medicine.anatomical_structure embryonic structures Genetics Osteocalcin biology.protein medicine Osteopontin Osteonectin Molecular Biology Progenitor
Zdroj:	DNA and Cell Biology. 12:871-880
ISSN:	1557-7430 1044-5498
DOI:	10.1089/dna.1993.12.871
Popis:	The cDNAs encoding the human bone morphogenetic proteins BMP-2 and BMP-4 in an eukaryotic expression vector were permanently transferred into the murine mesenchymal progenitor cell line C3H10T½. Originally, these cells are known to differentiate into myotubes, adipocytes, and chondrocytes upon the addition of azacytidine. Permanent transfection of genes encoding human BMP-2 and BMP-4 induces differentiation into the osteogenic lineage. The osteogenic differentiation potential of C3H10T½ cells is substantiated by histochemical and genetic analyses of marker genes typical or specific for osteogenesis, including the parathyroid hormone receptor, alkaline phosphatase, osteopontin, osteonectin, and osteocalcin. In addition to osteoblast formation, development into adipocytes and chondrocytes is also observed, suggesting that BMP-2 and BMP-4 induce differentiation into three mesenchymal lineages.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d04569ceb3a2bd296dc11fe69eafad38 https://doi.org/10.1089/dna.1993.12.871 Zobrazit plný text záznamu