Sequence of cDNA coding for a 65 kDa adhesive protein for the specific detection ofTrichomonas vaginalisby PCR

Autor:	Pietro Antonio Cappuccinelli, G Erre, Paola Rappelli, Angela Maria Rocchigiani, Mauro M. Colombo, Pier Luigi Fiori
Rok vydání:	1995
Předmět:	cDNA library Nucleic acid sequence Amplicon Biology medicine.disease_cause Microbiology Molecular biology law.invention law Complementary DNA Genetics medicine Recombinant DNA Trichomonas vaginalis Molecular Biology Gene Polymerase chain reaction
Zdroj:	FEMS Microbiology Letters. 129:21-26
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1995.tb07551.x
Popis:	A Trichomonas vaginalis cDNA library was constructed and recombinant plaques were screened using rabbit immunoglobulins specific for P65, a protozoan protein involved in pathogenicity that we identified in a previous study. A 1.38 kilobases cDNA fragment coding for the P65 protein was cloned in E. coli and then sequenced. On the basis of of the sequence obtained, six primers were synthesised and used to set up a Polymerase Chain Reaction. The presence of a specific amplicon in all 30 clinical isolates tested shows that P65 is a conserved and stable gene. The reaction is highly sensitive (as few as 5 to 10 parasites can be detected) and specific for Trichomonas vaginalis; the gene coding P65 adhesin can be therefore considered a very good molecular target for polymerase chain reaction-based diagnostic purposes.
Databáze:	OpenAIRE
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