The Gata1 murine megakaryocyte–erythroid progenitor cells expand robustly and alter differentiation potential
Autor: | Haiyoung Jung, Ji-Yoon Noh, Tae-Don Kim, Jong-Gwan Jeong, Eunju Shin, Charny Park, Inpyo Choi, Seung Jin Lee, Hyunmin Chung, Suk Ran Yoon |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Biophysics GATA1 Stem cell factor Cell Biology Biology Biochemistry Cell biology 03 medical and health sciences Haematopoiesis 030104 developmental biology 0302 clinical medicine Cell culture 030220 oncology & carcinogenesis Erythropoiesis Progenitor cell Molecular Biology Thrombopoietin Megakaryopoiesis |
Zdroj: | Biochemical and Biophysical Research Communications. 528:46-53 |
ISSN: | 0006-291X |
Popis: | GATA1 is a master transcription factor of megakaryopoiesis and erythropoiesis, and loss-of-function mutation can induce accumulation of megakaryocyte-erythroid progenitors (MEPs) in mice and humans. Accordingly, the murine MEP cell line (termed G1ME2 cells) encoding doxycycline (dox)-inducible anti-Gata1 shRNA on Hprt locus has been developed. The cells were CD41+CD71+KIT+, expand under dox, stem cell factor, and thrombopoietin (TPO), and terminally differentiate into erythroid cells or megakaryocytes upon removal of dox. Surprisingly, in this study, these Gata1low murine MEPs displayed accelerated growth from around 90-100 days after cell culture, impeded megakaryocytic potential, and maintained erythropoiesis. We specified them as late G1ME2 cells and discovered that increased CD41-KIT+ population during long-term culture was the main reason for the delayed megakaryopoiesis. The CD41 expression level was partially de-repressed by PI3K/AKT inhibitors, suggesting that TPO-mediated cell survival signaling pathway might have impacted on CD41 in the late G1ME2 cells. Nevertheless, among the late cells, the CD41+KIT+ cells could still generate megakaryocytes on dox withdrawal. Taken together, G1ME2 cells could provide a good model to study molecular mechanism of hematopoiesis because of their ability to expand excessively without artificial immortalization. |
Databáze: | OpenAIRE |
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