Inflammatory gene expression analysis after prebiotic, probiotic and synbiotic supplementation in experimental nonalcoholic fatty liver disease
| Autor: | Dan Linetzky Waitzberg, Odair Aguiar, Daniel Araki Ribeiro, Jessica Almeida da Cruz Ferreira, Claudia Cristina Alves, Angelica Carreira dos Santos |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
medicine.medical_specialty Nutrition and Dietetics 030309 nutrition & dietetics business.industry Prebiotic medicine.medical_treatment medicine.disease law.invention Pathogenesis 03 medical and health sciences Probiotic 0302 clinical medicine Endocrinology law Internal medicine Gene expression Expression analysis Nonalcoholic fatty liver disease medicine 030211 gastroenterology & hepatology Tumor necrosis factor alpha business Inflammatory genes Food Science |
| Zdroj: | Nutrition & Food Science. 49:75-84 |
| ISSN: | 0034-6659 |
| DOI: | 10.1108/nfs-04-2018-0119 |
| Popis: | Purpose The purpose of this study is to evaluate the effects of prebiotic, probiotic and synbiotic supplementation on liver histopathology and TLR-4, NFκB and TNF-α gene expression involved in the inflammatory cascade and pathogenesis of experimental nonalcoholic fatty liver disease (NAFLD). Design/methodology/approach Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (60 days). On Day 30 of hypercholesterolemic conditions, rats were subdivided in five groups: negative control (NC), positive control (PC), prebiotic (PRE), probiotic (PRO) and synbiotic (SYN). All rats were sacrificed on Day 60. Liver tissue was used to verify histopathological changes and gene expression. Gene expression of TLR-4, TNF-α and NFκB was evaluated in liver tissue using RT-qPCR. Findings Histopathological analysis: PC showed more changes than NC, and PRE and SYN showed fewer alterations than PC. Gene expression analysis: PRE showed higher TLR-4, and NFκB and TNF-α compared to PC. Also, PRE showed higher TLR-4 when compared to PRO and SYN. SYN group revealed higher TLR-4 and NFκB expressions compared to PC. PRO group also showed higher NFκB expression compared to PC. Originality/value NAFLD is a significant health concern, and it found that prebiotic, probiotic and synbiotic supplementation could have positive effects as a nonpharmacological approach to control this disease. |
| Databáze: | OpenAIRE |
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