Structure of the O-Polysaccharide of Providencia alcalifaciens O22 Containing D-Glyceramide 2-Phosphate

Autor:	Nina A. Kocharova, Magdalena Bialczak-Kokot, Alexander S. Shashkov, Olga G. Ovchinnikova, Antoni Rozalski, Yuriy A. Knirel
Rok vydání:	2012
Předmět:	Serotype chemistry.chemical_classification biology Chemistry Organic Chemistry Bacterial polysaccharide biology.organism_classification Phosphate Polysaccharide chemistry.chemical_compound Glyceramide Biochemistry Trisaccharide Physical and Theoretical Chemistry Providencia alcalifaciens Bacteria
Zdroj:	European Journal of Organic Chemistry. 2012:3500-3506
ISSN:	1434-193X
Popis:	O-Polysaccharides (O-antigens) are highly diverse glycopolymers present on the cell surface of Gram-negative bacteria, including the human opportunistic pathogen Providencia alcalifaciens. They define serospecificity of strains and are used for the serotyping of bacteria. In this work, a phosphorylated O-polysaccharide was isolated from P. alcalifaciens O22 and analyzed by chemical methods, ESI-MS, and 1H, 13C, and 31P NMR spectroscopy. It was found to contain two unusual components, 2-acetamido-4-amino-2,4,6-trideoxy-D-galactose (D-FucNAc4N) and D-glyceramide 2-phosphate (D-GroAN-2-P), the latter being identified for the first time in bacterial polysaccharides. The structure of the trisaccharide repeating unit of the O-polysaccharide was established to be 4)-(D-GroAN-2-P-3-)--D-GalNAc-(14)--D-Gal-(13)--D-FucNAc4N-(1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d01bca30486b85de7ac6a7ac03d6dfd4 https://doi.org/10.1002/ejoc.201200318 Zobrazit plný text záznamu Plný text