Determination of Anti-Pseudomonal Activities of Cocoa (Theobroma cacao) Pod Husk using Time-Kill-Test and Agar-Well Diffusion Techniques

Autor: M. F. Ikeola, O. A. Buhari, A. O. Issah, A. O. Boyejo, S. L. Owolabi, A.M. Deji- Agboola, I. A. Azeez
Rok vydání: 2021
Předmět:
Zdroj: Journal of Advances in Microbiology. :81-87
ISSN: 2456-7116
DOI: 10.9734/jamb/2021/v21i1230416
Popis: Aim: To determine the antimicrobial effectiveness of cocoa pod husk against P. aeruginosa. Study Design: An experimental research Place and Duration of Study: Department of Medical Microbiology, Olabisi Onabanjo University Teaching Hospital, Sagamu, Ogun State, Nigeria, between June 2015 and Sept. 2016. Materials and Methods: Seventy-seven (77) isolates of P. aeruginosa obtained from three (3) teaching hospitals were involved in this study. Cocoa pod husk was processed into crude extract and its effectiveness against the isolates of P. aeruginosa were examined using agar well diffusion, time-kill test techniques and Anova. Results: The percentage susceptibility of P. aeruginosa to Cocoa pod husk at 500 mg/mL and 250 mg/mL inhibited all the P. aeruginosa but only 14.29% were sensitive at a lower concentration of 31.25 mg/mL. Moreover, the comparison between the broth micro and macro dilution method of determining the MIC of cocoa pod husk against P. aeruginosa highlighted a significant decrease in the mean MIC value of the broth micro dilution method when compared with the broth macro dilution technique (T=13.519, P
Databáze: OpenAIRE