Emerging From the Unknown: Structural and Functional Features of Agnoprotein of Polyomaviruses
| Autor: | Serge Bouaziz, A. Sami Saribas, William E. Davis, Jon H. Condra, Mahmut Safak, Pascale Coric, Wayne E. Childers, Magid Abou-Gharbia, Rachael Axman, Anahit Hamazaspyan, Martyn K. White |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Regulation of gene expression Ku70 Physiology viruses Clinical Biochemistry Cell Cell Biology Protein phosphatase 2 Biology Molecular biology 3. Good health Proliferating cell nuclear antigen Cell biology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Transcription (biology) Phosphoprotein biology.protein medicine FEZ1 |
| Zdroj: | Journal of Cellular Physiology. 231:2115-2127 |
| ISSN: | 0021-9541 |
| Popis: | Agnoprotein is an important regulatory protein of polyomaviruses, including JCV, BKV, and SV40. In the absence of its expression, these viruses are unable to sustain their productive life cycle. It is a highly basic phosphoprotein that localizes mostly to the perinuclear area of infected cells, although a small amount of the protein is also found in nucleus. Much has been learned about the structure and function of this important regulatory protein in recent years. It forms highly stable dimers/oligomers in vitro and in vivo through its Leu/Ile/Phe-rich domain. Structural NMR studies revealed that this domain adopts an alpha-helix conformation and plays a critical role in the stability of the protein. It associates with cellular proteins, including YB-1, p53, Ku70, FEZ1, HP1α, PP2A, AP-3, PCNA, and α-SNAP; and viral proteins, including small t antigen, large T antigen, HIV-1 Tat, and JCV VP1; and significantly contributes the viral transcription and replication. This review summarizes the recent advances in the structural and functional properties of this important regulatory protein. J. Cell. Physiol. 231: 2115-2127, 2016. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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