Subcellular Distribution and Pharmacological Identification of M1 Receptors in the Submucosal Nasal Gland Acinar Cells of Guinea Pigs

Autor:	Tomonori Takasaka, Akira Shimomura, Narihisa Ueda, Seiichiro Nakabayashi, Katsuhisa Ikeda, Masayuki Furukawa, Dazheng Wu
Rok vydání:	1996
Předmět:	Pathology medicine.medical_specialty Subcellular distribution Otorhinolaryngology business.industry medicine Identification (biology) Muscarinic acetylcholine receptor M1 Nasal glands Anatomy business
Zdroj:	American Journal of Rhinology. 10:173-180
ISSN:	1539-6290 1050-6586
DOI:	10.2500/105065896781794905
Popis:	A muscarinic receptor in the submucosal nasal gland acinar cells controls electrolyte and fluid secretion. We investigated the pharmacological identification and the subcellular distribution of Ml receptor using a selective antagonist, pirenzepine (PZ). Intracellular Ca2+ concentration ([Ca2+]i) in the isolated acinar cells was measured by a fura-2 method. Addition of PZ inhibited the increase of the [Ca2+]i induced by acetylcholine in a dose-dependent manner, indicating the presence of the Ml receptor. Both fixed section of the nasal septal mucosa and living isolated acinar cells were selectively stained by fluorescence-labeled PZ. Furthermore, confocal laser scanning microscopy revealed that PZ specifically labeled the plasma membrane in the isolated acinar cells. Thus, M1 receptor was proven to be located in the plasma membrane of the submucosal nasal gland acinar cell.
Databáze:	OpenAIRE
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