| Popis: |
AIM: To study the mutation in a determinant of HBV S gene, to explore how it influenced the biologic characteristics of S gene. METHODS: Through site-mutation PCR, we constructed series variant clones (T126S, M133L, T144A) of HBV S gene ‘a’ determinant according to epidemiologic data. RESULTS: After analysis of sequence and expression of cells, sequences of mutation clone were correct and the mutation of different positions in ‘a’ determinant could influence antigenicity of expressed HBsAg. |
