Unique in utero identification of fetuses in multifetal mouse pregnancies by placental bidirectional arterial spin labeling MRI
| Autor: | Inbal E. Biton, Michal Neeman, Joel R. Garbow, Reut Avni, Vyacheslav Kalchenko, Tal Raz |
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| Rok vydání: | 2011 |
| Předmět: |
0303 health sciences
Fetus Pathology medicine.medical_specialty 030219 obstetrics & reproductive medicine Context (language use) Uterine horns Anatomy Biology Ovarian artery 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure In utero medicine.artery Placenta embryonic structures Knockout mouse medicine Radiology Nuclear Medicine and imaging Uterine artery 030304 developmental biology |
| Zdroj: | Magnetic Resonance in Medicine. 68:560-570 |
| ISSN: | 0740-3194 |
| DOI: | 10.1002/mrm.23246 |
| Popis: | Noninvasive imaging is a critical part of the study of developing embryos/fetuses, particularly in the context of alterations of gene expression in genetically modified animals. However, in litter-bearing animals, such as mice, the inability to accurately identify individual embryo/fetus in utero is a major obstacle to longitudinal, noninvasive in vivo studies. Arterial spin labeling MRI was adopted here to determine the fetal order along the uterine horns in vivo, based on the specific pattern of dual arterial blood supply within the mouse uterine horns. Blood enters the mouse uterus cranially through the ovarian artery and caudally through the uterine artery. Saturation slices were alternately placed on the maternal heart or on the bifurcation point of the common iliac artery, thereby saturating either downward inflow via the ovarian arteries or upward inflow via the uterine arteries, respectively. Saturation maps provided a unique signature with highly significant correlation between the direction-dependent magnetization transfer and the position of the fetuses/placentas along the uterine horns. The bidirectional arterial spin labeling-MRI method reported here opens possibilities to determine and pursue phenotypic alterations in fetuses and placentas in longitudinal studies of transgenic and knockout mice models, and for studying defects in placental vascular architecture. |
| Databáze: | OpenAIRE |
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