High-performance liquid chromatographic determination of amphotericin B in plasma and tissue

Autor:	Celia Barturen, M. J. Renedo, Maria del Carmen Dios-Viéitez, Iciar Echevarrı́a
Rok vydání:	1998
Předmět:	Chromatography Chemistry Organic Chemistry Kidney metabolism General Medicine Reversed-phase chromatography Piroxicam Biochemistry High-performance liquid chromatography Analytical Chemistry Pharmacokinetics Amphotericin B Blood plasma medicine Quantitative analysis (chemistry) medicine.drug
Zdroj:	Journal of Chromatography A. 819:171-176
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(98)00425-7
Popis:	A sensitive HPLC method with piroxicam as internal standard was developed for assaying amphotericin B in plasma and tissue. An Ultrabase-C18 column and a simple mobile phase consisting of an acetonitrile-acetic acid (10%)-water (41:43:16) mixture were used. The flow-rate was 1 ml/min and the effluent was monitored at 405 nm. The linearity of the assay method was up to 1000 ng/ml and 100 micrograms/g for plasma and tissue, respectively. Intra-day and inter-day RSDs were below 10% for all the sample types. This HPLC assay has been applied to determine amphotericin B in plasma and tissue samples taken during pharmacokinetic and tissue distribution studies in rats.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cff8678e806475d97dbafad4a89351e9 https://doi.org/10.1016/s0021-9673(98)00425-7 Zobrazit plný text záznamu Full Text from ScienceDirect