Nature of temperature gradients in capillary zone electrophoresis
| Autor: | Allan E. Jones, Eli Grushka |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Chromatography
Capillary action Chemistry Organic Chemistry Analytical chemistry General Medicine engineering.material Biochemistry Analytical Chemistry Physics::Fluid Dynamics Condensed Matter::Soft Condensed Matter Capillary electrophoresis Capillary column Coating Electrical resistivity and conductivity engineering High input Quartz Polyimide |
| Zdroj: | Journal of Chromatography A. 466:219-225 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(01)84618-5 |
| Popis: | An expression for the radial temperature profile in capillary zone electrophoresis was derived, taking into account the temperature dependence of the buffer electrical conductivity and the polyimide coating of the quartz capillary. Calculations show that in typical capillary zone electrophoresis experiments; i.e. , capillaries with 50–100 μm I.D., 375 μm O.D., and up to 5 W power input, the temperature profile rigorously derived is nearly identical to a parabolic profile. At high input powers, the parabolic approximation underestimates the temperature at the capillary center. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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