Single-cell profiling of tumor-infiltrating TCF1/TCF7+ T cells reveals a T lymphocyte subset associated with tertiary lymphoid structures/organs and a superior prognosis in oral cancer
| Autor: | Bowen Li, Lizao Zhang, Tianjun Lan, Siqi Ren, Jinsong Li, Niu Liu, Xinyu Lin, Qunxing Li, Yu Peng, Song Fan, Fan Wu, Zhanpeng Ou, Haixu Rong, Liping Xiao, Tingting Cai |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
medicine.diagnostic_test T cell Cell Cancer T lymphocyte Biology medicine.disease Flow cytometry 03 medical and health sciences 0302 clinical medicine Lymphatic system medicine.anatomical_structure Immune system Oncology Single-cell analysis 030220 oncology & carcinogenesis medicine Cancer research Oral Surgery 030223 otorhinolaryngology |
| Zdroj: | Oral Oncology. 119:105348 |
| ISSN: | 1368-8375 |
| Popis: | Objectives Despite substantial advances in treatment, clinical outcomes for oral squamous cell carcinoma (OSCC) remain unsatisfactory. Tumor-infiltrating lymphocytes (TILs) are an important prognostic factor for patients and are heterogeneous. Some studies have suggested that TCF1/TCF7+ T cells and tertiary lymphatic structure/organ (TLS) play an important role in tumor immunity. However, how they affect tumor immunity and whether they are related to prognosis in OSCC have not been reported in detail. Materials and methods We isolated OSCC cells and performed single-cell RNA sequencing (scRNA-seq). We used immunohistochemistry (IHC) to analyze the relationship between TLSs and prognosis. Multiplex immunohistochemistry (MIHC), flow cytometry (FCM) and spatial analysis were performed to verify the characteristics of TCF1/TCF7+ T cells. The prognostic significance and upstream regulatory network of the TCF1/TCF7+ T cell subpopulation were determined by multivariate analysis and Scenic software. Results We found a strong association between TCF1/TCF7+ T cell subsets, TLSs and prognosis. The results suggested that TCF1/TCF7+ T cells express high levels of TLS-related genes and low levels of immune checkpoint molecules. Finally, we found that TCF1/TCF7+ T cells were significantly associated with favorable outcomes. We also describe the upstream drivers that these cells rely on. Conclusions TCF1/TCF7+ T cells could be used as a new therapeutic target to regulate the immune response of OSCC and are expected to be a new prognostic marker. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect