Studies on the regeneration-restore and karyotype of protoplast in Metarhizium anisopliae var. majus

Autor:	Yun-gen Miao, L.-G. Shi
Rok vydání:	2005
Předmět:	fungi Metarhizium anisopliae Karyotype Fungi imperfecti Fungus Biology Protoplast biology.organism_classification Electrophoreses medicine.anatomical_structure Insect Science Botany Schizosaccharomyces pombe medicine Agronomy and Crop Science Nucleus
Zdroj:	Journal of Applied Entomology. 129:165-169
ISSN:	1439-0418 0931-2048
Popis:	The characteristics and regeneration-restore of protoplasts and its karyotype of an insect pathological fungus, Metarhizium anisopliae var. majus were studied. Among the protoplasts, 25.3% were without a nucleus, and 74.7% contained a nucleus. Among the nucleus protoplasts, 53.6% contained a single nucleus. The regeneration-restore of protoplasts was of three distinct shapes. Considering the frequency of regeneration and the growing speed of the colony, 0.7 mol/l glucose was the optimum as osmotic stabilizer of culture medium in the regeneration-restore of the protoplasts. The chromosomal DNA molecules of M. anisopliae var. majus have been separated into seven bands by pulsed-field gel electrophoreses. Using the Schizosaccharomyces pombe chromosomes as size standard, the size of chromosomal DNA was estimated to be 1.1–6.5 Mb and its karyotype exhibited polytypism among strains.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cfd4fa7f4c691c9fe27f5e477d7c89c6 https://doi.org/10.1111/j.1439-0418.2005.00946.x Zobrazit plný text záznamu Plný text