Estimation on metabolic pathway of Pseudomonas sp. SMIC-3 for 1-methyl-2-pyrrolidinone based on physiological and biochemical analyses

Autor:	Jun Yeong Yi, Doo Hyun Park, Bo Young Jeon
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Chromatography biology Chemistry Methylamine General Chemical Engineering Pseudomonas General Chemistry biology.organism_classification Catalysis Metabolic pathway chemistry.chemical_compound Enzyme Biochemistry Succinimide Dehydrogenation NAD+ kinase
Zdroj:	Korean Journal of Chemical Engineering. 31:475-484
ISSN:	1975-7220 0256-1115
DOI:	10.1007/s11814-013-0231-4
Popis:	Pseudomonas sp. SMIC-3 grown on NMP was physiologically differentiated from that on glucose. Growth of SMIC-3 in an NMP-defined medium was approximately three times lower than that in a glucose-defined medium. Methylamine and 1-methyl succinimide were detected in culture fluid of SMIC-3 grown in an NMP-defined medium. Methylamine content in the culture fluid was very similar to NMP consumed by SMIC-3, but 1-methyl succinimide content was much less than the consumed NMP. Crude enzyme isolated from SMIC-3 grown on NMP catalyzed production of methylamine, 1-methyl succinimide, and succinate from NMP but that on glucose did not. Crude enzyme isolated from SMIC-3 grown on glucose and NMP commonly catalyzed dehydrogenation of pyruvate, isocitrate, and malate coupled to reduction of NAD+ to NADH. 2D-SDS-PAGE pattern of total soluble proteins isolated from SMIC-3 grown on glucose was significantly different from that on NMP. Physiological function of SMIC-3 for catabolizing NMP may be selectively induced and activated by NMP.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cfd2ae3ab7df398a5beff4acd2cc77f4 https://doi.org/10.1007/s11814-013-0231-4 Zobrazit plný text záznamu Full text from SpringerLink