Bacterial endotoxin selectively prevents the expression of scavenger-receptor activity on human monocyte-macrophages
| Autor: | B J Van Lenten, A M Fogelman, J Seager, E Ribi, M E Haberland, P A Edwards |
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| Rok vydání: | 1985 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 134:3718-3721 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.134.6.3718 |
| Popis: | Concentrations of bacterial lipopolysaccharide (LPS) as low as 1 ng/ml suppressed the activity of the scavenger receptor on cultured human monocyte-macrophages. In contrast, concentrations of LPS as high as 100 ng/ml had no effect on the activity of the low density lipoprotein (LDL) receptor. LPS and purified forms of the lipid A moiety of LPS were effective in suppressing scavenger receptor activity. However, acid hydrolysis of the labile phosphate group of the native diphosphorylated lipid A to form monophosphoryl lipid A rendered the molecule ineffective in suppressing scavenger receptor activity. LPS at a concentration of 100 ng/ml had no effect on the secretion of apolipoprotein E, phagocytic activity, tumoricidal activity, or the protein content of monocyte-macrophages. We conclude that the active component of LPS that mediates suppression of scavenger receptor activity is diphosphoryl lipid A. |
| Databáze: | OpenAIRE |
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