Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR)
| Autor: | Elaine B. Shapland, Sunil S. Chandran |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology DNA synthesis FLP-FRT recombination Saccharomyces cerevisiae Computational biology biology.organism_classification Non-homologous end joining 03 medical and health sciences chemistry.chemical_compound Synthetic biology 030104 developmental biology chemistry DNA construct Homologous recombination DNA |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493963416 |
| DOI: | 10.1007/978-1-4939-6343-0_14 |
| Popis: | The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction. |
| Databáze: | OpenAIRE |
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