PECULIARITIES OF THE EFFECTS OF BILE ACIDS ON ATPASE ACTIVITY OF THE COLON MUCOSA IN PATIENTS WITH OVERWEIGHT AND IRRITABLE BOWEL SYNDROME
| Autor: | Mykola A Bychkov, Solomiia V. Bychkova, Iryna M. Ferents |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
biology business.industry ATPase General Medicine Overweight medicine.disease 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Endocrinology ATP hydrolysis 030220 oncology & carcinogenesis Internal medicine medicine Pi biology.protein Atpase activity 030211 gastroenterology & hepatology medicine.symptom business Reticulum Irritable bowel syndrome |
| Zdroj: | Wiadomości Lekarskie. 73:574-577 |
| ISSN: | 0043-5147 |
| DOI: | 10.36740/wlek202003133 |
| Popis: | OBJECTIVE The aim is to investigate the effect of bile acids on the ATPase activity of the colon mucosa in patients with overweight and irritable bowel syndrome (IBS). PATIENTS AND METHODS Materials and methods: Completely examined 12 patients with IBS and overweight.We estimated the ATPase activity of colon mucous of the patients with IBSspectrophotometrically by determined the content of orthophosphate that was released after ATP hydrolysis. We studied the effect of 3-sulphate of taurolitocholate (TLC-S) on specific activities of Na+/ K+-ATPase, Ca2+-ATPase of endoplasmatic reticulum (EPR),Ca2+-ATPase of plasmatic membrane (PM) and basal Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS. RESULTS Results: We establishedthe specific activities of Na+/K+-ATPase, Ca2+-ATPase of EPR,Ca2+-ATPase of PM and basal Mg2+-ATPase. Therewere(6.06 ± 1.61), (5.88 ± 1.19), (8.86 ± 1.56) (6.44 ± 2.02)µmol Pi/ mg protein per hour, respectively. TLC-S (50 µM) did not causedany change of Na+/K+-ATPase , as well as Ca2+-ATPasesactivities, but statistically significant increased activity of Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS by 4 fold. CONCLUSION Conclusions: TLC-S increased basal Mg2+-ATPase in the postmitochondrial fraction of colon mucous of the patients with overweight and IBS, but had no effect on Na+/K+-ATPase and Ca2+-ATPases activities. It has been suggested that activation of basal Mg2+-ATPase under by TLC-S may indicates the role of the endo-lysosomal system of epitheliocytes of colon mucous in developing of pathology IBS. |
| Databáze: | OpenAIRE |
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