Autor: M. Rozs, László Manczinger, Alejandro Hochkoeppler, A. G. Vara Y Rodríguez, F. Kevei, Cs. Vágvölgyi
Rok vydání: 2001
Předmět:
Zdroj: Biotechnology Letters. 23:1925-1929
ISSN: 0141-5492
DOI: 10.1023/a:1013746103442
Popis: A keratin-degrading strain of Bacillus licheniformis (K-508) was isolated from partially-degraded feathers and characterised. It had high chicken feather-degrading activity when cultured in feather-containing broth, with a growth optimum at pH 7 and 47 °C. Broth filtrates were active towards N-Bz-Phe-Val-Arg-p-nitroanilide and N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide, as chromogenic protease substrates at pH 8. Strain K-508 displays keratinolytic activity against native feather keratin (without any pretreatment) in the presence of SH-reducing compounds. It constitutively secreted both trypsin-like and chymotrypsin-like proteases.
Databáze: OpenAIRE