Autor: | M. Rozs, László Manczinger, Alejandro Hochkoeppler, A. G. Vara Y Rodríguez, F. Kevei, Cs. Vágvölgyi |
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Rok vydání: | 2001 |
Předmět: |
Proteases
animal structures Protease Bacillaceae biology Strain (chemistry) medicine.medical_treatment Bioengineering General Medicine biology.organism_classification Applied Microbiology and Biotechnology Bacillales Keratinase Biochemistry medicine biology.protein Fermentation Bacillus licheniformis Biotechnology |
Zdroj: | Biotechnology Letters. 23:1925-1929 |
ISSN: | 0141-5492 |
DOI: | 10.1023/a:1013746103442 |
Popis: | A keratin-degrading strain of Bacillus licheniformis (K-508) was isolated from partially-degraded feathers and characterised. It had high chicken feather-degrading activity when cultured in feather-containing broth, with a growth optimum at pH 7 and 47 °C. Broth filtrates were active towards N-Bz-Phe-Val-Arg-p-nitroanilide and N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide, as chromogenic protease substrates at pH 8. Strain K-508 displays keratinolytic activity against native feather keratin (without any pretreatment) in the presence of SH-reducing compounds. It constitutively secreted both trypsin-like and chymotrypsin-like proteases. |
Databáze: | OpenAIRE |
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