Boromycin Has Potent Anti- Toxoplasma and Anti- Cryptosporidium Activity
| Autor: | Roberta M. O'Connor, Alexis Cotto-Rosario, Jaypee Abenoja |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pharmacology medicine.diagnostic_test biology 030106 microbiology Toxoplasma gondii Cryptosporidium Immunofluorescence biology.organism_classification medicine.disease Toxoplasmosis Microbiology 03 medical and health sciences Boromycin chemistry.chemical_compound 030104 developmental biology Infectious Diseases Cryptosporidium parvum Antigen chemistry parasitic diseases medicine Parasite hosting Pharmacology (medical) |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 65 |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | Toxoplasma gondii and Cryptosporidium parvum, members of the phylum Apicomplexa, are significant pathogens of both humans and animals worldwide for which new and effective therapeutics are needed. Here, we describe the activity of the antibiotic boromycin against Toxoplasma and Cryptosporidium Boromycin potently inhibited intracellular proliferation of both T. gondii and C. parvum at half-maximal effective concentrations (EC50) of 2.27 nM and 4.99 nM, respectively. Treatment of extracellular T. gondii tachyzoites with 25 nM boromycin for 30 min suppressed 84% of parasite growth, but T. gondii tachyzoite invasion into host cells was not affected by boromycin. Immunofluorescence of boromycin-treated T. gondii showed loss of morphologically intact parasites with randomly distributed surface antigens inside the parasitophorous vacuoles. Boromycin exhibited a high selectivity for the parasites over their host cells. These results suggest that boromycin is a promising new drug candidate for treating toxoplasmosis and cryptosporidiosis. |
| Databáze: | OpenAIRE |
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