Bioprocess Scale-up for Acetohydroxamic Acid Production by Hyperactive Acyltransferase of Immobilized Rhodococcus Pyridinivorans

Autor: Pradeep Kumar, Jeevan Lata, Nandita Thakur, Duni Chand, Deepak Pandey, Neena Devi, Sanjay K.S. Patel, Vikram Thakur, Alok Singh
Rok vydání: 2021
Předmět:
Zdroj: Catalysis Letters. 152:944-953
ISSN: 1572-879X
1011-372X
DOI: 10.1007/s10562-021-03696-4
Popis: In this study, Rhodococcus pyridinivorans cells containing hyperactive acyltransferase was immobilized on various macromolecules based-polymeric matrices and used to improve acetohydroxamic acid production. The calcium-alginate-based matrix retained the maximum residual activity up to 97.8% as compared to free cells (576 U/mg of dry cell weight). After immobilization, cells exhibited a significant improvement in their tolerance towards pH, temperature, and metal ions as potent enzyme inhibitors. Immobilized cells showed 25.5-fold higher thermal stability at 60 °C to control (free cells). Compared to free cells, immobilized cells exhibited a high bioconversion of acetamide and hydroxylamine-HCl to acetohydroxamic acid up to 96% molar conversion. Repeated bench-scale production at 3-L culture, immobilized cells showed 9.5-fold higher residual conversion as compared to control (100%), after five cycles of reuses. The product characterization achieved high purity (97%) of acetohydroxamic acid. This finding showed high feasibility to achieve efficient conversion that can be scaled up to the industrial level for biotechnological application.
Databáze: OpenAIRE
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