| Popis: |
Medicinal plant products are generally used as immunomodulatory (stimulatory/suppressive/adjuvant) agents for curing various ailments. These activities could be due to the presence of primary and secondary metabolites as already described in the literature. In the present study, we focused on those proteases isolated from the flowers of Caesalpinia pulcherrima and Phanera variegata against specific protein antigen i.e. Bovine serum albumin (BSA) on human diabetic blood samples using flow cytometry. For these studies, determination of protein (Nanodrop) and protease (spectropho-tometer) content from the flowers of Caesalpinia pulcherrima and Phanera variegata using phosphate buffered saline (PBS, pH 3 to 9). Out of these, the most active protease treated with diabetic human blood samples (n=6; fasting glucose level, >180) using diluted and concentrated form of protease (100 µl) and determined its blood counts in the form of lymphocytes, monocytes and granulocytes count and also analysed its forward and side scatter of the cell. The results showed that acid protease (pH 5) showed enhancement in granulocytes count but sudden decline in monocytes count as compared to control. Similarly, there is enhancement in both forward and side scatter after treating with acid protease of Caesalpinia pulcherrima and Phanera variegata. Overall, flow cytometric results revealed that acid protease (pH 5) from the flowers of Caesalpinia pulcherrima and Phanera variegata showed antidiabetic property in human blood samples. |
