A novel solid-phase synthetic method for production of N -alkyl-4-alkylamino-1-aryl-1 H -pyrazolo[3,4- d ]pyrimidine-6-carboxamide library

Autor: Yun-Jeong Heo, Moon-Kook Jeon
Rok vydání: 2017
Předmět:
Zdroj: Tetrahedron. 73:5959-5973
ISSN: 0040-4020
DOI: 10.1016/j.tet.2017.08.044
Popis: This work describes a solid-phase synthetic method for building a compound library of N -alkyl-4-alkylamino-1-aryl-1 H -pyrazolo[3,4- d ]pyrimidine-6-carboxamide derivatives, that are based on the biologically active 1-aryl-1 H -pyrazolo[3,4- d ]pyrimidine scaffold. In the first step of this synthetic sequence, condensation reactions of ethyl 5-amino-1-aryl-1 H -pyrazole-4-carboxylates with methyl cyanoformate resulted in the formation of esters that underwent hydrolysis to give 1-aryl-4,5-dihydro-1 H -pyrazolo[3,4- d ]pyrimidin-4-one-6-carboxylic acids. The coupling reaction of these carboxylic acids with primary alkylamine-loaded acid-sensitive methoxybenzaldehyde (AMEBA) resins was followed by amination reactions mediated by benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate (BOP). Subsequent cleavage from the solid support resulted in the formation of the target N -alkyl-4-alkylamino-1-aryl-1 H -pyrazolo[3,4- d ]pyrimidine-6-carboxamide derivatives. The reaction conditions for solid-phase transformations were optimized using a solution-phase model study with 2,4-dimethoxybenzyl-protected isobutylamine as a reactant in place of the AMEBA resin-loaded isobutylamine. The progress of the solid-phase reactions was monitored by on-bead ATR-FTIR spectroscopy. Diversification experiments were performed by using 1-aryl-4,5-dihydro-1 H -pyrazolo[3,4- d ]pyrimidin-4-one-6-carboxylic acids and a variety of primary and secondary amine building blocks to build the target compound library.
Databáze: OpenAIRE
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