Kinetically inert manganese (II) -based hybrid micellar complexes for magnetic resonance imaging of lymph node metastasis

Autor: Kai Chen, Zhongyuan Cai, Yingzi Cao, Lingling Jiang, Yuting Jiang, Haojie Gu, Shengxiang Fu, Chunchao Xia, Lui Su, Qiyong Gong, Bin Song, Hua Ai
Rok vydání: 2023
Předmět:
Zdroj: Regenerative Biomaterials.
ISSN: 2056-3426
2056-3418
DOI: 10.1093/rb/rbad053
Popis: The localization and differential diagnosis of the sentinel lymph nodes (SLN) are particularly important for tumor staging, surgical planning, and prognosis. In this work, kinetically inert manganese (II) -based hybrid micellar complexes (MnCs) for magnetic resonance imaging were developed using an amphiphilic manganese-based chelate (C18-PhDTA-Mn) with reliable kinetic stability and self-assembled with a series of amphiphilic PEG-C18 polymers of different molecular weights (C18En, n = 10, 20, 50). Among them, the probes composed by 1:10 mass ratio of manganese chelate/C18En had slightly different hydrodynamic particle sizes with similar surface charges as well as considerable relaxivities (∼13 mM−1s−1 at 1.5 T). In vivo lymph node imaging in mice revealed that the micellar complex MnC-20 formed by C18E20 with C18-PhDTA-Mn at a hydrodynamic particle size of 5.5 nm had significant signal intensity brightening effect and shortened T 1 relaxation time. At an imaging probe dosage of 125 μg Mn/kg, lymph nodes still had significant signal enhancement in 2 hours, while there is no obvious signal intensity alteration in non-lymphoid regions. In 4T1 tumor metastatic mice model, sentinel lymph nodes showed less signal enhancement and smaller T 1 relaxation time variation at 30 min post injection, when compared with normal lymph nodes. This was favorable to differentiate normal lymph nodes from SLN under a 3.0 T clinical MRI scanner. In conclusion, the strategy of developing manganese-based MR nanoprobes was useful in lymph node imaging.
Databáze: OpenAIRE
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