Actinomycetes from Moroccan habitats: isolation and screening for cytotoxic activities

Autor:	A. Chait, M. Taourirt, A. Zyad, M. Anibou, A. Benherref, Yedir Ouhdouch
Rok vydání:	2008
Předmět:	biology Physiology Gram-positive bacteria General Medicine biology.organism_classification Applied Microbiology and Biotechnology Streptomyces Microbiology Cytotoxic T cell Artemia salina Antibacterial activity Cytotoxicity Bacteria Biotechnology Antibacterial agent
Zdroj:	World Journal of Microbiology and Biotechnology. 24:2019-2025
ISSN:	1573-0972 0959-3993
DOI:	10.1007/s11274-008-9705-7
Popis:	In our screening for actinomycetes showing cytotoxic activities, 8 samples were collected from various Moroccan habitats, 136 isolates were tested for their capacity to produce antibacterial compounds against gram positive bacteria. Thirty-seven strains of these isolates were active against Gram-positive bacteria. Using the following steps of primary screening: antibacterial activity, confrontation between the isolates and toxicity to Artemia salina; fifteen different isolates were used for further investigation. The aqueous extracts of Streptomyces sp. T5 and Streptomyces sp. AS8 were selected for their cytotoxic activity against Hep2, BSR and P815 cell lines, and two active compounds were observed on HPLC. The two isolates exhibited high activity against human cancer cell lines and were inactive on PBMC cell lines. Furthermore, the Streptomyces sp. T5 extract showed a proliferative activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cf86ffae149c692014c83e28486a1241 https://doi.org/10.1007/s11274-008-9705-7 Zobrazit plný text záznamu Full text from SpringerLink