Characterization of the okra mucilage by interaction with Gal, Ga1NAc and GlcNAc specific lectins

4Gal sequence, which is the ligand for the uropathogenic Escherichia coli and toxic lectins. Analysis of the binding property of the okra polysaccharide by precipitin assay with Gal, GalNAc and GlcNAc specific lectins showed that this okra mucilage reacted best with Mistletoe toxic lectin-I (ML-I) and precipitated over 80% of the ML-I nitrogen (5.1 micrograms N) added. It also precipitated well with Abrus precatorius (APA), Momordica charantia (MCA) and Ricinus communis (RCA1) agglutinins, but poorly with other lectins. The results obtained suggest that this polysaccharide is a valuable reagent to differentiate Gal specific lectins from the GalNAc and/or GlcNAc specific series. -->
ISSN: 0304-4165
DOI: 10.1016/0304-4165(94)00130-p
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=doi_________::cf6cc381253c9ab67fda9c1277f76b11
https://doi.org/10.1016/0304-4165(94)00130-p
Rights: CLOSED
Přírůstkové číslo: edsair.doi...........cf6cc381253c9ab67fda9c1277f76b11
Autor: F-shiun Shen, Pei-Yi Hwang, Yia-jen Jiang, Albert M. Wu
Rok vydání: 1995
Předmět:
Zdroj: Biochimica et Biophysica Acta (BBA) - General Subjects. 1243:157-160
ISSN: 0304-4165
DOI: 10.1016/0304-4165(94)00130-p
Popis: A bio-active polysaccharide, which was the major component of the extract of the common okra, Hibiscus esculentus, was isolated from the extract by precipitation with ethanol between 28.5 to 45%. According to a previous report (Whistler, R.L. and Conrad, H.E. (1954) J. Am. Chem. Soc. 76, 1673-1674), this polysaccharide contains the Gal alpha 1-->4Gal sequence, which is the ligand for the uropathogenic Escherichia coli and toxic lectins. Analysis of the binding property of the okra polysaccharide by precipitin assay with Gal, GalNAc and GlcNAc specific lectins showed that this okra mucilage reacted best with Mistletoe toxic lectin-I (ML-I) and precipitated over 80% of the ML-I nitrogen (5.1 micrograms N) added. It also precipitated well with Abrus precatorius (APA), Momordica charantia (MCA) and Ricinus communis (RCA1) agglutinins, but poorly with other lectins. The results obtained suggest that this polysaccharide is a valuable reagent to differentiate Gal specific lectins from the GalNAc and/or GlcNAc specific series.
Databáze: OpenAIRE
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