Confirmation of an Enzyme-Linked Immunosorbent Assay to Detect Fluometuron in Soil1

Autor: Michele Boyette, David R. Shaw, Mark W. Shankle
Rok vydání: 2001
Předmět:
Zdroj: Weed Technology. 15:669-675
ISSN: 1550-2740
0890-037X
DOI: 10.1614/0890-037x(2001)015[0669:coaeli]2.0.co;2
Popis: Research was conducted to compare the results of an enzyme-linked immunosorbent assay (ELISA) to high-performance liquid chromatography (HPLC) for detecting fluometuron in the environment. A linear relationship for HPLC (R2 > 0.90) and ELISA (R2 > 0.66) analysis was observed between the natural logarithm of the detected fluometuron concentrations regressed against time in soil collected from a cropped area, a grass filter strip, and a riparian forest. Both methods detected the same initial fluometuron concentration (y-intercept) for two of the three soils evaluated. The ELISA and HPLC measurements of fluometuron concentrations compared favorably with r values from 0.83 to 0.98. Predicted fluometuron half-lives determined from HPLC and ELISA measurements were: 110 and 112 d in the cropped watershed, 28 and 29 d in the riparian area, and 11 and 11 d in the grass filter strip, respectively. Results from both techniques indicated shorter half-lives in soil from the grass filter strip and riparian area than in...
Databáze: OpenAIRE