An evaluation of the endocrine disruptive potential of crude oil water accommodated fractions and crude oil contaminated surface water to freshwater organisms using in vitro and in vivo approaches
| Autor: | Lucky Mokwena, Paul J Oberholster, Johannes H. van Wyk, J. Christoff Truter, Anna-Maria Botha |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Amphibian African clawed frog Oreochromis mossambicus food.ingredient biology Health Toxicology and Mutagenesis Deiodinase Tilapia Fuel oil 010501 environmental sciences biology.organism_classification 01 natural sciences Thyroid hormone receptor beta 03 medical and health sciences 030104 developmental biology food biology.animal Environmental chemistry biology.protein Freshwater fish Environmental Chemistry Food science 0105 earth and related environmental sciences |
| Zdroj: | Environmental Toxicology and Chemistry. 36:1330-1342 |
| ISSN: | 0730-7268 |
| DOI: | 10.1002/etc.3665 |
| Popis: | Knowledge regarding the potential impacts of crude oil on endocrine signalling in freshwater aquatic vertebrates is limited. In this study, the expression of selected genes as biomarkers for altered endocrine signalling were studied in African clawed frog, Xenopus laevis tadpoles, and juvenile Mozambique tilapia, Oreochromis mossambicus exposed to weathered bunker and un-weathered refinery crude oil water accommodated fractions (WAFs). In addition, the expression of the aforementioned genes was quantified in X. laevis tadpoles exposed to surface water collected from the proximity of an underground oil bunker. The (anti)estrogenicity and (anti)androgenicity of crude oil, crude oil WAFs and surface water were furthermore evaluated using recombinant yeast. Thyroid hormone receptor beta expression was significantly downregulated in X. laevis in response to both oil WAF types, whereas, a further thyroid linked gene, type 2 deiodinase was upregulated in O. mossambicus exposed to a high concentration of bunker oil WAF. In addition, both WAFs altered the expression of the adipogenesis-linked peroxisome proliferator-activated receptor gamma in X. laevis. The crude oil and WAFs exhibited anti-estrogenic and anti-androgenic activity in vitro. However, O. mossambicus androgen receptor 2 was the only gene, representing the reproductive system, significantly affected by WAF exposure. Estrogenicity, anti-estrogenicity and anti-androgenicity were detected in surface water samples; however, no significant changes were observed in the expression of any of the genes evaluated in X. laevis exposed to surface water. The responses varied among the two model organisms used, as well as among the two types of crude oil. Nonetheless, our data provide evidence that crude oil pollution may lead to adverse health effects in freshwater fish and amphibians due to altered endocrine signalling. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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