Development of an in vitro System for the Evaluation of Allelopathic Activities of Asparagus Calluses

Autor:	Sumio Iwai, Yasuki Watanabe, Yoshiharu Fujii, Yoshitaka Ono, Syuntaro Hiradate, Fuminori Komai
Rok vydání:	2011
Předmět:	biology fungi Cell volume food and beverages musculoskeletal system biology.organism_classification body regions Horticulture surgical procedures operative In vitro system Callus Botany Bioassay Asparagus Aseptic processing Sexual difference Allelopathy
Zdroj:	Journal of the Japanese Society for Horticultural Science. 80:82-88
ISSN:	1882-336X 1882-3351
Popis:	An aseptic bioassay system to estimate allelopathy in asparagus calluses was established. Allelopathic activities of calluses were measured according to the callus packed cell volume (PCV) by means of the sandwich method, and remarkable inhibition of acceptor plants was observed by the addition of over 0.1 mL PCV. When female and male calluses were separately used for the sandwich method, there was no sexual difference in allelopathic activity. To detect the allelopathic activities of the callus cells in detail, we improved the plant box method that substituted the calluses for the seedlings, and serious inhibition of acceptor plants was observed with the addition of over 0.35 mL PCV as donor material. Allelopathic activities were accelerated by irradiating the calluses with light-emitting diodes (LEDs) prior to performing the aseptic bioassay. It also seemed that the light quality would affect their activities. The allelopathic activity appeared intensely with irradiation of the calluses with a blue LED. The aseptic bioassay system that employed LED-irradiated calluses established here could accelerate effectively the isolation and identification of allelochemical(s).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cf5bcd1e730a5eb3bfdb846710d1a1be https://doi.org/10.2503/jjshs1.80.82 Zobrazit plný text záznamu