Marker-assisted pyramiding of Xa21 and Xa7 genes conferring resistance to bacterial leaf blight in indica cultivar Bacthom7
| Autor: | Nguyen Viet Long, Vu Hong Quang, Tran Thi Thu Hoai, Nguyen Thi Mien, Tran Thi Lien, Vu Äuc Lam, Vu Van Liet, Nguyen Thi Hue, Nguyen Thi Thu, Nong Thi Hue, Mai Van Tan, Nguyen Van Hoan, Nakano Toshitsugu, Nguyen Dinh Trung |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Resistance (ecology) Inoculation food and beverages Biology biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences Horticulture 030104 developmental biology Xanthomonas oryzae Genetics Grain quality Blight Paddy field Cultivar Agronomy and Crop Science Molecular Biology Gene 010606 plant biology & botany Biotechnology |
| Zdroj: | African Journal of Biotechnology. 17:1389-1396 |
| ISSN: | 1684-5315 |
| DOI: | 10.5897/ajb2017.16059 |
| Popis: | Bacterial leaf blight (BLB) of rice is one of the most destructive diseases affecting rice fields. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of BLB. Two BLB resistance genes, Xa21 and Xa7, were transferred into the susceptible indica cultivar Bacthom7 (BT7) by using marker-assisted selection with markers pTA248 for Xa21 and ID7 for Xa7. Improved BT7 lines carrying the two resistance genes were inoculated with three isolates of the Xoo from Northern Vietnam and evaluated for agronomic traits. Artificial inoculation of 13 lines with three Xoo races identified nine highly resistant lines with wide-spectrum resistance to Xoo, including D1, D2, D3, D6, D7, D8, D9, D10 and D12. These lines were similar to recurrent parent BT7 with regard to external appearance, yield performance and grain quality. On the basis of agronomic traits and the level of resistance to BLB, two promising lines, D6 and D9 were further selected. These two lines could efficiently contribute to rice production for food security and food safety in northern Vietnam. Key words: Xanthomonas oryzae pv. oryzae, resistance genes, near-isogenic lines, marker-assisted selection (MAS), improved rice lines. |
| Databáze: | OpenAIRE |
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