Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation

Autor:	Maud A. W. Hinchee, Sherri M. Brown, C. G. Ford-Santino, Michael E. Fromm, David L. DeBoer, R. B. Horsch, D. D. Songstad, Charles L. Armstrong, F. G. Halaka
Rok vydání:	1993
Předmět:	chemistry.chemical_classification Reporter gene Electroporation fungi food and beverages Embryo Horticulture Biology Plant cell Molecular biology chemistry.chemical_compound Enzyme chemistry Anthocyanin Gene expression Botany DNA
Zdroj:	Plant Cell, Tissue and Organ Culture. 33:195-201
ISSN:	1573-5044 0167-6857
DOI:	10.1007/bf01983234
Popis:	Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 µF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cf581598ebe0f37c44ef88389776b7a6 https://doi.org/10.1007/bf01983234 Zobrazit plný text záznamu Full text from SpringerLink